Methodologies

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30

45Ca Uptake
39

Acetone
2

Alizarin stain
28

Alkalinity Anomaly Technique (Smith and Key 1975)
3

Archimedes principle
48

Ash free dry weight (AFDW)
43

Bicinchoninic acid assay
46

Bradford 1976
29

Buoyant weight
1

CSF imaging
57

CT Scanning
10

Calculated from GIS shapefile
74

Calculated from Veron ecoregion shapefile
4

Callipers
33

Chloroform to methanol ratio
52

Cloning
40

Counted doublet with cell plate in samples of cells
42

DC Protein Assay Al Foil Marsh 1970
51

DGGE
34

Decalcified tissues measured with calipers
6

Depth gauge
9

Derived from range map
22

Derived from textual description
14

Dissection
49

Dried at 80 degrees C
50

Dried for 24 h at 40 degrees C
77

Dry density
11

Extracted from Coral ID digital compact disk
23

Extracted from data table
75

Faunal break cluster analysis
63

Filter combustion
62

Flow injection analyser
69

Fluorescence assay (relative quantities of RNA and DNA)
17

Fluorescence microscope
44

Formalin/acid
37

Frozen, airbrush
73

Genus age estimate
72

Growth form Mizerek grouping protocol
55

Growth form derived from Veron (2000) textual descriptions
56

Growth form derived from Wallace et al (2012) textual descriptions
78

Growth rate estimated from length-age growth functions
38

Hemocytometer counts
13

Histology
15

Image analysis
65

Irradiometer
5

Kobe porosimeter
31

Linear extension alizerin staining and calipers, bulk density wax dipping and buoyant weight
45

Lowery et al 1951
70

MCMC phylogenetic tip length
35

Measured on cross sections using stereomicroscope
36

Measured with callipers
16

Microscopy
58

Observation_field_or_laboratory
25

Oxygen consumption in chambers in the dark
24

Oxygen consumption in dark stirring chamber normalized to surface area by foil
26

Oxygen consumption in the dark in small flume
61

Oxygen meter
27

Oxygen production in the light in stirring chambers
68

PDZ Europa ANCA-GSL elemental analyzer
32

Product of linear extension and bulk density
66

Rain gauge
76

Random forests analyses (Darling et al. 2012)
59

Refractometer
54

Ruler measurement
60

Secchi disk
64

Sediment trap
53

Sequencing
12

Timed distance
7

Transect tape
41

Two counts from fluorescent images on hemocytometer
18

Weight
8

X-radiography (densitometer)
21

inferred
19

observation_field
20

observation_laboratory
67

on release

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